HPLC and UHPLC Unveiled: Mastering Chromatography for Pharma and Biotech in 2025
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HPLC and UHPLC Unveiled: Mastering Chromatography for Pharma and Biotech in 2025
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James Carter
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Elevate your analytical expertise with HPLC and UHPLC Unveiled: Mastering Chromatography for Pharma and Biotech in 2025, a comprehensive guide crafted for pharmaceutical and biotechnology professionals. Spanning nine in-depth chapters, this resource delivers over 200 technical strategies to optimize lab workflows—from High-Performance Liquid Chromatography (HPLC)’s foundational 400-bar, 5-micron separations ensuring 99.8% purity in 10 minutes to Ultra-High-Performance Liquid Chromatography (UHPLC)’s 1,500-bar, sub-2-micron precision resolving biologics at 0.001% in 5 minutes. Trace the evolution from HPLC’s 1970s roots to UHPLC’s dominance—70% adoption by 2024, per BioPharma Trends—driven by biologics (40% of 2025 pipelines). Learn to scale methods with precision, troubleshoot pressure spikes in 15 minutes, and harness AI tools boasting 95% accuracy for GMP compliance and R&D breakthroughs. Covering small molecules, biologics, and chiral drugs, this book equips you to triple throughput to 100 samples per day, meeting ICH Q3A’s stringent <0.05% impurity thresholds. Explore 2025 trends shaping the future—automation (55% adoption), sustainable green solvents (30% of runs), advanced columns (65%), AI integration (40%), portable UHPLC (15%), and hybrid UV-MS detection (50%, 0.001% sensitivity)—positioning labs for innovation. Whether you’re a novice mastering retention times or a veteran refining gradients, this guide bridges HPLC’s reliability (15% of runs) with UHPLC’s cutting-edge standard (projected 75% by 2027). Packed with actionable insights—method optimization, peak resolution (Rs > 2.5), and biologics analysis—this is your essential companion for achieving analytical excellence in 2025’s demanding landscape. Dive in to sharpen your skills and propel lab performance forward.
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